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KMID : 0357319680030010015
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Journal of the Korean Society for Microbiology
1968 Volume.3 No. 1 p.15 ~ p.23
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Studies on the Rapid Plasma Reagin(RPR) Card Test for the Diagnosis of Syphilis
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金周德/Kim, Joo Deuk
柳駿/金顯周/Lew, Joon/Kim, Hyun Joo
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Abstract
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For the effective control of Syphilis, many investigators have developed a more rapid, simple: and economical screening serological test which is adequately sensitive and specific.
To fulfill the requirements of a more rapid serologic test for syphilis, a substitute for thee conventional serum specimen was needed since considerable time and labor are involved in the processing of blood to serum.
Burdon suggested the use of plasma in the serologic tests for syphilis as a substitute for serum. He noticed that plasma was more sensitive than serum in the Kline and Kahn tests, and. . attributed this to the presence of more antibody-like substance, "reagin" in plasma than in serum. However, to make plasma sufficiently sensitive, it was necessary to inactivate plasma by heating at a temperature of b6°C for about 30 minutes. Heating of plasma resulted in thee precipitation of fibrinogen which made centrifugation -necessary to obtain clear plasma. Since the chief disadvantage to the use of unheated plasma (or serum) was a reduction in sensitivity of results-which probably was due to a labile factor such as complement-Portnoy et al began to.. consider rapid chemical methods of inactivation of plasma (or serum). They experienced that choline chloride was shown to be anticomplementary which suggested its use as an inactivating agent for unheated plasma (or serum). In 1959 Portnoy et al reported the Rapid Plasma Reagin. (RPR) Test for syphilis which is a more rapid, economical and simple. But still this test has. many disadvantages as a rapid performing, field and o face procedure, because it requires the. usual laboratory equipments such as centrifuge, rotating machine, microscope etc.
To substitute these disadvantages of the RPR test, in 1962, Portnoy et al developed the Rapid Plasma Reagin (RPR) card test for syphilis, which has the following advantages:
a) Simplicity and rapidity of performance,
b) Requires no laboratory equipments,
c) Stable antigen suspension,
d) Adequate sensitivity and specificity.
This RPR card test can be used as a rapidly performing and screening test in field investiga-
would present that the two tests, RPR card and VDRL, are similar in sensitivity.
Comparing the results of RPR card, VDRL and RPCF tests (table 4), it showed higher percentage of agreement (81.6%) between RPR card and RPCF tests than agreement (75.5%) between VDRL and RPCF tests. Therefore, it would mean that RPR card test is more specific than VDRL test.
There has been a need for a rapid simple office and field testing procedure for the diagnosis of syphilis in Korea. The RPR card test performed with a small amount of plasma obtained from plasma collection slide might be of valuable tool for office and field testing procedure for the diagnosis of syphilis, and as an adjunct in planning for syphilis control in Korea.
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